Hybridization probe size control: optimized ‘oligolabelling’

PROBE: a Metaheuristic for Hybridization Extended Abstract

a ribo probe and its application for iranian leshmania hybridization

introduction: lishmania is a protozoan parasite that cause visceral and cutaneous disease in man. this parasite shows two forms in its life cycle, amastigote in reticulo-endothelial cell of mammalian host and, promastigote in culture media and sand fly vector. like other kinetoplastide, leishmania has an extra chromosomal dna with unique topological and characteristic that called kdna. the orga...

Radiationless deactivation of hybridization-sensitive DNA probe

In this study we attempt to determine the deactivation mechanism of a hybridization-sensitive DNA probe by using steady state absorption and fluorescence spectroscopy. Thiazole orange or 2,20Methylenebis(N-alkylbenzothiazole) is covalently linked to a single nucleotide in the DNA probe. Radiationless deactivation of the fluorophores is mainly determined by photo-induced isomerization of the met...

development and implementation of an optimized control strategy for induction machine in an electric vehicle

in the area of automotive engineering there is a tendency to more electrification of power train. in this work control of an induction machine for the application of electric vehicle is investigated. through the changing operating point of the machine, adapting the rotor magnetization current seems to be useful to increase the machines efficiency. in the literature there are many approaches wh...

Counting and size classification of active soil bacteria by fluorescence in situ hybridization with an rRNA oligonucleotide probe.

A fluorescence in situ hybridization (FISH) technique based on binding of a rhodamine-labelled oligonucleotide probe to 16S rRNA was used to estimate the numbers of ribosome-rich bacteria in soil samples. Such bacteria, which have high cellular rRNA contents, were assumed to be active (and growing) in the soil. Hybridization to an rRNA probe, EUB338, for the domain Bacteria was performed with a...